Polypeptide N-acetylgalactosaminyltransferase-15 regulates adipogenesis in human SGBS cells

Adipogenesis involves intricate molecular mechanisms regulated by various transcription factors and signaling pathways. In this study, we aimed to identify factors specifically induced during adipogenesis in the human preadipocyte cell line, SGBS, but not in the mouse preadipocyte cell line, 3T3-L1. Microarray analysis revealed distinct gene expression profiles, with 1460 genes induced in SGBS cells and 1297 genes induced in 3T3-L1 cells during adipogenesis, with only 297 genes commonly induced. Among the genes uniquely induced in SGBS cells, we focused on GALNT15, which encodes polypeptide N-acetylgalactosaminyltransferase-15. Its expression increased transiently during adipogenesis in SGBS cells but remained low in 3T3-L1 cells. Overexpression of GALNT15 increased mRNA levels of CCAAT-enhancer binding protein (C/EBPα) and leptin but had no significant impact on adipogenesis in SGBS cells. Conversely, knockdown of GALNT15 suppressed mRNA expression of adipocyte marker genes, reduced lipid accumulation, and decreased the percentage of cells with oil droplets. The induction of C/EBPα and peroxisome proliferator-activated receptor γ during adipogenesis was promoted or suppressed in SGBS cells subjected to overexpression or knockdown of GALNT15, respectively. These data suggest that polypeptide N-acetylgalactosaminyltransferase-15 is a novel regulatory molecule that enhances adipogenesis in SGBS cells.

factors, specifically the CCAAT-enhancer binding protein (C/EBP) family and peroxisome proliferator-activated receptor γ (PPARγ), are crucial 2,3 .C/EBPβ and C/EBPδ are transiently induced early in adipogenesis 4 and they induce expressions of C/EBPα and PPARγ, which function to promote transcription of many types of genes involved in adipocyte phenotype and function 2,3 .In addition to these key transcriptional regulators, various other transcriptional regulators of adipogenesis and factors affecting adipogenesis have been reported.While many of these findings regarding adipogenesis have been obtained using in vitro mouse models utilizing cell lines, such as 3T3-L1 or 3T3-F442A, the mechanisms of adipogenesis in humans and mice are not entirely identical.For instance, 3T3-L1 and 3T3-F442A cells undergo an increase in cell number known as mitotic clonal expansion prior to differentiation, which is thought to be necessary for subsequently differentiation.However, human primary preadipocytes or SGBS cells, a human preadipocyte cell line established from the subcutaneous adipose tissue of an infant suspected of having Simpson-Golabi-Behmel Syndrome 5 , do not necessarily undergo mitotic clonal expansion during adipogenesis 6,7 .Furthermore, LIM domain only 3 (LMO3), which positively regulates adipogenesis, is upregulated during adipogenesis in humans but not in mice 8 .Additionally, D-dopachrome tautomerase, an adipokine secreted by adipocytes, suppresses adipogenesis in SGBS cells but not in 3T3-L1 cells 9 .Thus, identifying the factors that affect adipogenesis in a human-specific manner may lead to the identification of novel target molecules for the development of anti-obesity drugs.
In the present study, we conducted a comparative analysis of mRNA expression induced during adipogenesis in SGBS cells and 3T3-L1 cells using microarray to identify genes whose expression is induced in SGBS but not in 3T3-L1 cells.GALNT15, encoding polypeptide N-acetylgalactosaminyltransferase (GalNAc-T)-15, was identified as one of the candidate genes.GALNT15, a member of the GALNT family comprising 20 species in humans, is expressed in most human tissues 10 .GalNAc-T15 catalyzes the initiation of mucin-type O-linked glycosylation by adding N-acetylgalactosamine to the serine or threonine residues of polypeptides 10 .Mucin-type O-glycosylation is initiated and regulated by the GalNAc-T family that catalyzes the first step in the biosynthesis forming the GalNAcα1-O-serine/threonine linkage in O-glycoproteins.O-linked glycosylation, the most diverse form of post-translational modifications, affects various aspects of protein function, therefore, many GalNAc-Ts are considered to have potentials for differential regulation in cells and tissues 11 .Aberrant O-glycosylation by some GalNAc-Ts has been observed in many types of cancer and is associated with noncancerous developmental and metabolic disorders 12,13 ; however, the involvement of GALNT15 in these diseases has not been reported, and its physiological function remains largely unknown.Therefore, we focused on GALNT15 and investigated its effects on adipogenesis in SGBS cells.

GALNT15 expression was induced during adipogenesis in SGBS but not in 3T3-L1 cells
Within our experimental setup, the mRNA expression of fatty acid binding protein 4, an adipocyte marker, has been peaked around day 7 in both 3T3-L1 and SGBS cells after adipogenic induction (Supplementary Fig. S1).Therefore, the mRNA expression profiles of SGBS and 3T3-L1 cells on days 0, 1, 3, and 7 after adipogenic induction were analyzed using microarray analysis.Genes whose expression was upregulated by more than 2-fold compared with that in cells before adipogenic induction (day 0) were extracted.In SGBS cells, 565, 880, and 955 genes were upregulated on days 1, 3, and 7, respectively, after adipogenic induction (Fig. 1A).Excluding overlapping genes, 1460 genes were identified as induced during adipogenesis in SGBS cells.These genes were further classified as "early" (peak on day 1), "late" (peak on day 3), and "gradual" (expression continuing up to day 7), resulting in 436 "early", 265 "late", and 579 "gradual" genes identified as being induced during adipogenesis in SGBS cells.Similarly, in 3T3-L1 cells, 807, 662, and 903 genes were induced on days 1, 3, and 7 after adipogenic induction, respectively (Fig. 1B), resulting in 1297 induced genes during adipogenesis and classified as 185 "early", 291 "late", and 460 "gradual" genes.In both cell types, only 297 genes were found to be commonly induced (Fig. 1C), which included well-known adipocyte marker genes, such as ADIPOQ (adiponectin), CEBPA (C/ EBPα), DGAT2 (diacylglycerol O-acyltransferase 2), FABP4 (fatty acid binding protein 4), and PPARG (PPARγ) (Table 1).Among the genes induced exclusively in SGBS cells, 61 exhibited more than 20-fold induction, including LMO3, whose expression is reported to be induced during adipogenesis in humans but not in mice 8 .Among these, we focused on GALNT15 due to its relatively high expression at day 0, large variation in expression levels, and its poorly understood function in most organs, including adipose tissue.Furthermore, considering that other members of the GALNT family were not induced during adipogenesis in either SGBS cells or 3T3-L1 cells (Supplementary Table S1), we speculated that GALNT15 may have a specific role in adipocyte differentiation.Microarray analysis revealed that the signal intensity of GALNT15 in SGBS cells increased 63.73-, 37.05-, and 1.30-fold on days 1, 3, and 7 after adipogenic induction, respectively (Table 1).By contrast, the signal intensity of Galnt15 in 3T3-L1 cells showed minor fluctuations, with fold changes of 1.02, −1.07, and 1.09, respectively, at the same time points following adipogenic induction.Quantitative reverse transcription polymerase chain reaction (qRT-PCR) validated these findings, highlighting a transient increase in GALNT15 mRNA levels during adipogenesis in SGBS but not in 3T3-L1 cells (Fig. 2A).Western blotting also confirmed a similar pattern, with GalNAc-T15 protein levels transiently tending to increase in SGBS cells but barely detected throughout adipogenesis in 3T3-L1 cells (Fig. 2B).
GALNT15 is an "early" gene whose expression may be influenced by components present in the medium used for adipogenic induction in SGBS cells but absent in the medium used for adipogenic induction in 3T3-L1 cells.Therefore, we investigated which components of the medium used for adipogenic induction in SGBS cells affect GALNT15 mRNA expression.The absence of fetal bovine serum (FBS) and glucocorticoids such as cortisol and dexamethasone (DEX) increased GALNT15 mRNA expression (Supplementary Fig. S2).

GALNT15 overexpression enhances CEBPA and LEP mRNA expression in SGBS cells
To investigate the impact of GALNT15 on adipogenesis in SGBS cells, we first constructed an adenovirus overexpressing the GalNAc-T15-FLAG fusion protein and assessed adipogenesis in SGBS cells transfected with this virus compared with that in cells transfected with a control virus (vehicle).Western blotting demonstrated the ectopic expression of the GalNAc-T15-FLAG fusion protein in SGBS cells (Fig. 3A).GALNT15 overexpression significantly increased the mRNA expressions of CEBPA and LEP, but not those of ADIPOQ, FABP4, and PPARG , in SGBS cells 7 days after adipogenic induction (Fig. 3B).The amount of triglyceride accumulation and Identification of genes with increased expression during adipogenesis in SGBS and 3T3-L1 cells using microarray.The numbers represent the count of genes that increased more than 2-fold after adipogenic induction at day 1, 3, and 7, compared with that before induction (day 0) in SGBS cells (A) and in 3T3-L1 cells (B).Genes are classified into "early", "late", and "gradual" based on their expression induction patterns during adipogenesis, with the number of genes in each category also shown.(C) A Venn diagram illustrating the overlap between number of genes upregulated more than 2-fold after adipogenic induction at either day 1, 3, or 7 compared with that before induction in SGBS cells and in 3T3-L1 cells.

GALNT15 knockdown inhibits adipogenesis in SGBS cells
Subsequently, we constructed adenoviruses expressing a short hairpin RNA (shRNA) against GALNT15 mRNA (shGALNT15) and a non-targeting control shRNA (shNC) and assessed adipogenesis in transfected SGBS cells.The induction of GALNT15 mRNA and protein expressions during adipogenesis was inhibited in SGBS cells transfected with the adenovirus expressing shGALNT15 (Fig. 4A, B).GALNT15 knockdown suppressed mRNA expression of all tested adipocyte marker genes (Fig. 4C), as well as the protein expression of adiponectin, C/ EBPα, and PPARγ (Fig. 4D), triglyceride accumulation (Fig. 4E, F), and the percentage of cells with oil droplets (Fig. 4G, H), indicating that GALNT15 knockdown inhibited adipogenesis in SGBS cells.

GALNT15 enhances the induction of PPARG and CEBPA during adipogenesis
To elucidate the molecular mechanisms by which GALNT15 participates in adipogenesis, we examined the mRNA levels of two key adipogenic transcriptional regulatory genes, PPARG and CEBPA, during adipogenesis.GALNT15 overexpression significantly enhanced the induction levels of both PPARG and CEBPA mRNA during adipogenesis in SGBS cells (Fig. 5A), whereas GALNT15 knockdown suppressed the induction of PPARG and CEBPA mRNA 4 days after adipogenic induction compared with that in control cells (Fig. 5B).These results indicate that GALNT15 is involved in adipogenesis by enhancing the expression of PPARG and CEBPA during adipogenesis in SGBS cells.Furthermore, the effect of GALNT15 on the mRNA expressions of CEBPB, a transcription factor upstream of PPARG and CEBPA, in the early stages of adipogenesis was investigated.Neither overexpression nor knockdown of GALNT15 altered the expression of CEBPB mRNA in SGBS cells at 0, 3, 6, 12 and 24 h after adipogenic induction (Fig. 5C, D), suggesting that mechanisms other than inducing the expression of CEBPB are involved in the regulation of PPARG and CEBPA by GALNT15.

Discussion
Microarray analysis revealed distinct expression patterns of many genes during adipogenesis in human SGBS cells and mouse 3T3-L1 cells.This may be attributed to species differences between humans and mice; however, there may also be other differences in their properties as preadipocytes.For instance, the profile of genes expressed by differentiated adipocytes derived from 3T3-L1 cells is markedly different from that expressed by mature adipocytes in mouse 15 .By contrast, the mRNA expression profile of adipocytes derived from SGBS cells is similar to that of primary human white subcutaneous adipocytes [16][17][18] .Nevertheless, numerous molecules implicated in adipogenesis have been identified and validated using 3T3-L1 cells.Thus, identifying genes induced during the adipogenic process in SGBS cells, but not in 3T3-L1 cells in this study, could serve as a viable strategy for identifying adipogenic factors as yet undiscovered in humans.In fact, among the 61 genes exhibiting more than 20-fold induction during the SGBS adipogenic process, but not in 3T3-L1 cells, some genes, except for GALNT15, whose involvement in adipogenesis is unknown to our knowledge, were included.Notably, with LMO3, a humanspecific adipogenic gene 8 , being one of these 61 genes, there is a possibility that unidentified adipogenic factors may also be present within this group.In this study, GALNT15 is identified as a gene induced during the adipogenesis of SGBS cells, consistent with reports indicating that GALNT15 is listed as one of upregulated during adipocyte differentiation from human adipose-derived stem cells 19 .The induction of GalNAc-T15 protein during adipogenesis in SGBS cells was less pronounced compared to the induction of GALNT15 mRNA, possibly due to post-transcriptional modifications, translation efficiency, mRNA stability, or other factors.However, the exact mechanisms are not extensively www.nature.com/scientificreports/covered in this study.Although the mRNA expression of Galnt15 was not induced, and protein expression was not detected during adipogenesis of 3T3-L1 cells, it cannot be ruled out that the induction was undetectable due to extremely low expression levels compared with those in SGBS cells.The regulation of Galnt15 gene expression is influenced by corticosterone and the stress response in the mouse hippocampus 20 .There is a distinction in the presence of FBS between the media employed for adipogenic induction of SGBS cells and 3T3-L1 cells.While FBS deficiency increased GALNT15 mRNA expression, probably due to cell stress, glucocorticoids appear to have a stronger impact on GALNT15 expression.Despite the presence of DEX in the medium used for adipogenic induction in 3T3-L1 cells, no induction of Galnt15 expression was observed during the adipogenic process, suggesting that the transcriptional regulation of GALNT15/Galnt15 might differ between humans and mice.This study focused on the identification of novel human adipogenesis-related factors, therefore, the investigation in mice was limited to confirming Galnt15 expression in 3T3-L1 cells.However, it is necessary to carefully consider whether a transient increase in the expression of Galnt15 is observed during mouse adipogenesis or whether Galnt15 is also involved in mouse adipogenesis.
Although inhibition of GALNT15 induction clearly impeded adipogenesis in SGBS cells, overexpression of GALNT15 did not affect the accumulation of triglycerides or the proportion of cells containing lipid droplets, mRNA expression after adipogenic induction at 0, 1, 3, and 7 days in SGBS cells (black columns) and 3T3-L1 cells (white columns).Data represents relative values compared with those before adipogenesis in each cell type.*P < 0.05 (n = 3).(B) Representative western blotting images using lysates from SGBS and 3T3-L1 cells subjected to adipogenic induction for the indicated days, probed with an anti-GalNAc-T15 antibody.Antiadiponectin antibody and anti-β-actin antibodies were used as positive controls for adipogenesis and internal controls, respectively.The graph illustrates the average band density of GalNAc-T15 normalized to β-actin in SGBS cells from four western blotting, with day 0 set to 1 as relative values.There were no statistically significant differences observed.
Vol:.( 1234567890 www.nature.com/scientificreports/despite affecting the induction of PPARG mRNA 4 days after adipogenic induction in SGBS cells.This suggests that the induced expression levels of PPARG during the adipogenic process in control SGBS cells may be sufficient to affect adipogenesis under our experimental conditions, with further overexpression potentially having no additional effect on these aspects.Furthermore, overexpression of GALNT15 enhanced only the mRNA expression of CEBPA and its direct target gene LEP 21 , among the tested adipogenic marker genes.The role of CEBPA in adipogenesis is limited to the induction and maintenance of PPARG expression and the establishment of insulin sensitivity 22 .This suggests that the lack of adipogenic promotion in SGBS cells overexpressing GALNT15 could be attribute to sufficient levels of PPARG expression.Conversely, GALNT15 knockdown may affect adipogenesis by suppressing CEBPA expression, resulting in insufficient PPARG expression levels for adipogenic differentiation.Additionally, the high basal levels of GalNAc-T15 in SGBS cells may explain the absence of adipogenic promotion observed with GALNT15 overexpression.Western blotting revealed the presence of GalNAc-T15 protein in SGBS cells prior to adipogenic induction, suggesting that the endogenous levels of GalNAc-T15 might be sufficient to support adipogenesis, even in the absence of exogenous GALNT15 overexpression.On the other hand, shGALNT15 likely reduced both the basal and induced levels of GalNAc-T15 during adipogenesis, strongly providing clearer insights into the functional role of GALNT15 in adipogenesis.
Abnormal O-GalNAc-glycosylation catalyzed by the GALNT family is associated with various human diseases, with particular attention focused on the link between GALNT2 and metabolic disorders, such as obesity, type 2 diabetes, and lipid abnormalities 23 .In vitro analysis has shown that a reduction in GALNT2 expression in HepG2 cells, a human hepatocarcinoma cell line, impairs insulin signaling and action 24 .Conversely, GALNT2 overexpression stimulates adipocyte maturation and enlargement in 3T3-L1 cells 25 .However, our microarray data revealed that, except for GALNT15, other GALNT family members did not exhibit a remarkable increase during the adipogenic process in SGBS cells.This may suggest a more profound role for GALNT15 than for GALNT2 in human adipogenesis.GALNT15 does not show a significant relation with other GALNT family member 11 , and to our knowledge, its physiological function has not been thoroughly investigated.Our findings, combined with the fact that GALNT15 also serves as a marker gene candidate during osteocyte differentiation from canine adipose derived stem cells 26 , suggest that GALNT15 may play an important role in the differentiation of mesenchymal stem cells.
In conclusion, we have demonstrated that GALNT15 contributes to adipogenesis in SGBS cells by upregulating CEBPA and PPARG .However, the specific molecular mechanisms driving GALNT15 -induced adipogenesis, including the potential involvement of unidentified substrates or non-enzymatic functions of GalNAc-T15, remain unclear and require further investigation for comprehensive elucidation.Our findings suggest that GALNT15 is an attractive drug target for the treatment of obesity.
3T3-L1 cells were generously provided by Oral Bioscience Laboratory, Tokushima University.Japan.The cells were cultured in 6-well plates with culture medium consisting of Dulbecco's modified Eagle's medium (Fujifilm) supplemented with 10% FBS and 0.5% penicillin-streptomycin-amphotericin B suspension.To induce adipogenesis, cells grown to 100% confluency were cultured for an additional 2 days and then cultured in medium containing 10 μM insulin, 1 µM DEX, 500 µM 3-isobutyl-1-methylxanthine, and 2 µM troglitazone for 3 days.Subsequently, the cells were cultured in maintenance medium consisting of medium containing 10 μM insulin.The maintenance medium was changed every 3 days.

Microarray
Total RNA was extracted from SGBS and 3T3-L1 cells before (day 0) and on days 1, 3, and 7 after adipogenic induction, using ISOGEN (Nippongene, Toyama, Japan).The extracted RNA was used to generate biotin-labeled cRNA using the Affymetrix GeneChip TM 3′ IVT PLUS Reagent Kit (Thermo Fisher Scientific, Waltham, MA, USA).The biotin-labeled RNA was then hybridized to either an Affymetrix Human Genome U-219 Array plate (Thermo Fisher Scientific) or a mouse genome MG-430 PM array plate (Thermo Fisher Scientific) following the manufacturer's instructions.After washing and staining the array strips, the signals were developed and scanned using the Affymetrix Gene Atlas system (Thermo Fisher Scientific), and the data were analyzed using Transcriptome Analysis Console software (Thermo Fisher Scientific).Average hybridization signal intensities were used for data analysis, and genes with a mean signal intensity greater than 5 (log base 2 scale) in either of the adipogenicinduced samples were considered detectable.Genes with a signal intensity more than 2-fold higher than that in each cell before adipogenic induction were considered induced genes, and comparisons were made between SGBS and 3T3-L1 cells.Genes with the same symbol in humans and mice were designated as common genes.

qRT-PCR
Each cDNA was synthesized from total RNA using the ReverTra Ace ® qPCR RT Kit (Toyobo, Osaka, Japan) following the manufacturer's protocol.The cDNA was then subjected to qRT-PCR on a Thermal Cycler Dice ® Real Time System (Takara, Shiga, Japan) using Thunderbird TM SYBR ® qPCR Mix (Toyobo) and gene-specific primer sets via the following program: 30 sec at 95 °C, followed by 40 cycles of 95 °C for 15 sec and 60 °C for 1 min.The specificity of each primer set was confirmed by dissociation curve analysis following amplification.The nucleotide sequences of the primer sets are listed in Supplementary Table S2.The mRNA level of each gene was normalized to that of the human and mouse glyceraldehyde 3-phosphate dehydrogenase gene (GAPDH/Gapdh).

Construction of adenoviruses
Adenoviruses expressing GalNAc-T15 fused to FLAG at the C-terminus and a shGALNT15 were constructed as previously described 14 .Briefly, cDNA encoding the translational region lacking the stop codon of GALNT15 was amplified from SGBS adipocyte cDNA using PCR, and DNA with FLAG cDNA sequences added to its 3'-end was inserted into the pAxCAwtit cosmid vector (TakaRa).Recombinant adenoviral genomic DNA was excised from the cosmid and transfected into HEK293 cells to produce an adenovirus.An adenovirus produced from intact pAxCAwtit was used as a control.Cosmids for adenovirus production, which were inserted with a cDNA encoding shGALNT15 (target sequence: 5'-AGT CTG CTC TCA GCG AAT ATG-3' , vector ID: VB900074-6932bqd) and a cDNA encoding a shNC (target sequence: 5'-CCT AAG GTT AAG TCG CCC TCG,-3' vector ID: VB010000-9479yzr), were purchased from Vector Builder (Chicago, IL, USA), and adenoviruses were produced in the same manner.

Evaluation of adipogenesis
The degree of adipocyte differentiation was evaluated based on the expression of adipocyte marker genes, triglyceride accumulation, and the percentage of cells with oil droplets.SGBS cells were infected with each adenoviruses for 24 h and subjected to adipogenic induction.Seven days after adipogenic induction, total RNA was extracted from the cells and the expression of adipocyte marker genes was measured by qRT-PCR.Ten days after adipogenic induction, SGBS cells were fixed with 4% paraformaldehyde and stained with Oil Red O to evaluate triglyceride accumulation or 4,6-diamidine-2-phenylindole dihydrochloride and Sudan III to count cells with oil droplets.To measure the amount of triglycerides, stained Oil Red O was eluted with isopropanol, and the absorbance was measured at 500 nm using a spectrophotometer (Ultrospec 6300 pro; GE Healthcare, Chicago, IL, USA).To assess the percentage of cells with oil droplets, the ratio of Sudan III-positive cells to 4,6-diamidine-2-phenylindole dihydrochloride-stained cells was determined in 3 randomly selected low-power fields (x100).Fig. 4. Effects of GALNT15 knockdown on adipogenesis in SGBS cells.SGBS cells were infected with adenovirus expressing shRNA against GALNT15 mRNA (shGALNT15; black columns) or control adenovirus expressing negative control shRNA (shNC; white columns) for 24 h, followed by adipogenic induction.Adipogenesis was evaluated on day 7 (C, D) or day 10 (E-H) after adipogenic induction.(A, B) Suppression of GALNT15 mRNA (A) and GalNAc-T15 (B) expressions by shGALNT15 during adipogensis in SGBS cells was confirmed by quantitative reverse transcription polymerase chain reaction (A) and western blotting (3T3-L1 cells), respectively.The graph represents relative values compared with those before adipogenesis in control cells.*P < 0.05 (n = 3).(C) mRNA expression of adipocyte marker genes in SGBS cells subjected to adipogenic induction.The graph represents relative values compared with those in control cells.*P < 0.05 (n = 3).(D) Representative western blotting images using lysates from SGBS cells subjected to adipogenic induction, probed with an anti-adiponectin, anti-C/EBPα, and anti-PPARγ antibodies.Anti-β-actin antibody was used as an internal control.
Fig.1.Identification of genes with increased expression during adipogenesis in SGBS and 3T3-L1 cells using microarray.The numbers represent the count of genes that increased more than 2-fold after adipogenic induction at day 1, 3, and 7, compared with that before induction (day 0) in SGBS cells (A) and in 3T3-L1 cells (B).Genes are classified into "early", "late", and "gradual" based on their expression induction patterns during adipogenesis, with the number of genes in each category also shown.(C) A Venn diagram illustrating the overlap between number of genes upregulated more than 2-fold after adipogenic induction at either day 1, 3, or 7 compared with that before induction in SGBS cells and in 3T3-L1 cells.

Fig. 2 .
Fig.2.GALNT15/Galnt15 expression during adipogenesis in SGBS and 3T3-L1 cells.(A) GALNT15/Galnt15.mRNA expression after adipogenic induction at 0, 1, 3, and 7 days in SGBS cells (black columns) and 3T3-L1 cells (white columns).Data represents relative values compared with those before adipogenesis in each cell type.*P < 0.05 (n = 3).(B) Representative western blotting images using lysates from SGBS and 3T3-L1 cells subjected to adipogenic induction for the indicated days, probed with an anti-GalNAc-T15 antibody.Antiadiponectin antibody and anti-β-actin antibodies were used as positive controls for adipogenesis and internal controls, respectively.The graph illustrates the average band density of GalNAc-T15 normalized to β-actin in SGBS cells from four western blotting, with day 0 set to 1 as relative values.There were no statistically significant differences observed.

)Fig. 3 .
Fig.3.Effects of GALNT15 overexpression on adipogenesis in SGBS cells.SGBS cells were infected with adenovirus overexpressing GalNAc-T15-FLAG fusion protein (GALNT15; black columns) or adenovirus without insertion (vehicle; white columns) for 24 h, followed by adipogenic induction.Adipogenesis was evaluated on day 7 (B) or day 10 (C-F) after adipogenic induction.(A) The expression of GalNAc-T15-FLAG fusion protein during adipogenesis in SGBS cells.Cell lysate from SGBS cells before the adenovirus infection (-1 day) and at the indicated day after adipogenic induction were probed with an anti-FLAG antibody using western blotting.An anti-β-actin antibody was used as an internal control.(B) mRNA expression of adipocyte marker genes in SGBS cells subjected to adipogenic induction.The graph represents relative values compared with those in control cells.*P < 0.05 (n = 3).(C, D) Lipid accumulation in SGBS cells subjected to adipogenic induction.Representative images (C) and the colorimetric quantification (D) in SGBS cells stained with Oil Red O are shown.N = 3. (E, F) Percentage of cells with oil droplets in SGBS cells subjected to adipogenic induction.Representative images (E) and graph of percentage (F) in SGBS cells stained both by 4,6-diamidine-2-phenylindole dihydrochloride (DAPI) and Sudan III are presented.The graph represents data averaged across three different fields (x100) of view.

Fig. 5 .
Fig.4.Effects of GALNT15 knockdown on adipogenesis in SGBS cells.SGBS cells were infected with adenovirus expressing shRNA against GALNT15 mRNA (shGALNT15; black columns) or control adenovirus expressing negative control shRNA (shNC; white columns) for 24 h, followed by adipogenic induction.Adipogenesis was evaluated on day 7 (C, D) or day 10 (E-H) after adipogenic induction.(A, B) Suppression of GALNT15 mRNA (A) and GalNAc-T15 (B) expressions by shGALNT15 during adipogensis in SGBS cells was confirmed by quantitative reverse transcription polymerase chain reaction (A) and western blotting (3T3-L1 cells), respectively.The graph represents relative values compared with those before adipogenesis in control cells.*P < 0.05 (n = 3).(C) mRNA expression of adipocyte marker genes in SGBS cells subjected to adipogenic induction.The graph represents relative values compared with those in control cells.*P < 0.05 (n = 3).(D) Representative western blotting images using lysates from SGBS cells subjected to adipogenic induction, probed with an anti-adiponectin, anti-C/EBPα, and anti-PPARγ antibodies.Anti-β-actin antibody was used as an internal control.(E, F) The effect on lipid accumulation in SGBS cells subjected to adipogenic induction was assessed by Oil Red O staining.Representative images (E) and the colorimetric quantification (F) of SGBS cells stained by oil red O are shown.*P < 0.05 (n = 3).(G, H) The effect on the percentage of cells with oil droplets in SGBS cells subjected to adipogenic induction.Representative images (G) and the percentage (H) of SGBS cells stained with both 4,6-diamidine-2-phenylindole dihydrochloride (DAPI) and Sudan III are presented.*P < 0.05 (n = 3).

Table 1 .
Fold changes and classification of microarray probeset signals for representative genes induced at day 1, 3, and 7 after adipogenic induction.